Enhancing protein self-association at the gas-liquid interface for foam fractionation of bovine serum albumin from its highly diluted solution

- Na-citrate interacted with tyrosine residues to unfold the BSA structure.
- It induced the formation of 12 and 18 BSA aggregates at the gas-liquid interface.
- The enhanced BSA-BSA association improved the stability of BSA foam.
- With 30 mmol/L Na-citrate, the BSA recovery increased by 4 folds.