کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
701296 1460819 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Adsorption and hydrolytic activity of lysozyme on diamond nanocrystallites
موضوعات مرتبط
مهندسی و علوم پایه سایر رشته های مهندسی مهندسی برق و الکترونیک
پیش نمایش صفحه اول مقاله
Adsorption and hydrolytic activity of lysozyme on diamond nanocrystallites
چکیده انگلیسی

Oxidative-acid-treated nanodiamonds exhibit high affinity for proteins, a property well suited for immobilization of enzymes for biotechnological application. Using lysozyme as an example, this work demonstrates that the enzyme can retain much of its activity after physical adsorption to the surfaces of 100-nm diamond crystallites. The activity relative to that of free lysozyme in solution is ∼ 60% at the maximum surface coverage of 50% and pH 5. While the enzymatic activity decreases as the surface coverage is lowered, it can be recovered by blocking the empty sites on the surface with supplementary proteins such as cytochrome c to create a more “crowded” environment. A relative activity up to 70% can be attained at a partial coverage of 20%.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Diamond and Related Materials - Volume 16, Issues 4–7, April–July 2007, Pages 872–876
نویسندگان
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