Synthesis and in vitro anticoagulation activity of hollow carbon nitride microspheres

Hollow carbon nitride microspheres have been synthesized by a facile templating method through replicating the spherical cavity of the porous silica. Different from the common closed hollow microspheres, the microspheres have one or more small holes on the surface, which may be more convenient for its use as small containers for drug delivery, encapsulation, etc., because the small holes can be used as the entrance for adding drug, proteins, enzymes, etc. The diameters of the hollow carbon nitride microspheres were about 1.8–2.2 μm, and the shell thickness of the hollow carbon nitride microspheres was about 70–100 nm. The composition and structure of the sample were detected by the X-ray powder diffraction (XRD), elemental analysis, Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectra (XPS). The in vitro anticoagulation activity of the sample was evaluated by the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT).