کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7138721 | 1462016 | 2018 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Highly efficient ratiometric extracellular oxygen sensors through physical incorporation of a conjugated polymer and PtTFPP in graft copolymers
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Highly efficient ratiometric extracellular oxygen sensors through physical incorporation of a conjugated polymer and PtTFPP in graft copolymers Highly efficient ratiometric extracellular oxygen sensors through physical incorporation of a conjugated polymer and PtTFPP in graft copolymers](/preview/png/7138721.png)
چکیده انگلیسی
An amphiphilic graft copolymer (P3) composed of poly(oligo (ethylene glycol) methacrylate) (POEGMA) as a hydrophilic segment and a polystyrene (PS) as a hydrophobic chain was prepared. P3 was used to incorporate a highly efficient hydrophobic oxygen probe platinum(II)-5,10,15,20-tetrakis-(2,3,4,5,6-pentafluorophenyl)-porphyrin (PtTFPP) in its micelles to enable the application of PtTFPP in aqueous solution for biosensing. For achieving ratiometric oxygen sensing, a hydrophobic conjugated polymer (CP) was used as a fluorescence resonance energy transfer (FRET) donor for PtTFPP - the FRET acceptor. Results showed that diameters of these nano-oxygen sensors ranging from 50 to 60â¯nm and FRET efficiency as high as 98% could be achieved. Further, FRET contributed to the brightness of PtTFPP in micelles. High quantum yields of PtTFPP in non-FRET micelles and FRET micelles under nitrogen were achieved to be 0.109 and 0.231, respectively. The sensors were found to be able to measure oxygen concentrations from 0.082 to 40.9â¯mg/L at room temperature with linear Stern-Volmer constants. These sensors were also demonstrated to be suitable for monitoring extracellular oxygen consumption of E. Coli and HeLa cells. This study illustrated a feasible approach to achieve highly efficient ratiometric oxygen sensors without specific structure modification of the efficient PtTFPP probe, however using an amphiphilic graft copolymer approach.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 273, 10 November 2018, Pages 242-252
Journal: Sensors and Actuators B: Chemical - Volume 273, 10 November 2018, Pages 242-252
نویسندگان
Qian Zhao, Tingting Pan, Gang Xiang, Zhipeng Mei, Jiapei Jiang, Gang Li, Xianshao Zou, Meiwan Chen, Dazhi Sun, Shimei Jiang, Yanqing Tian,