کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7142436 | 1462053 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A colorimetric paper sensor for lactate assay using a cellulose-Binding recombinant enzyme
ترجمه فارسی عنوان
یک حسگر کاغذ رنگی برای آزمایش لاکتات با استفاده از یک آنزیم نوترکیب سلولز اتصال دهنده
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A new fabrication technology for effective preparation of paper-based biosensors using affinity-binding enzymes is developed. Lactate dehydrogenase (LDH) fused with a cellulose binding domain (CBD) is produced to allow one-step purification and immobilization of the enzyme on paper. Results demonstrate that the binding capacity of the recombinant enzyme is as high as 22Â mg-enzyme/g-paper, with much improved stability (by a factor of 25-fold) in comparison to the native parent enzyme. That ensures the solid phase bioassay reactions on surface of the paper sensor are not limited by enzyme availability, and affords much extended sensor shelf life at the same time. The paper sensor shows a linear detection range of 0.5-8Â mM of lactate, with changes in color intensity detectable within a much broader concentration range examined (up to 16Â mM). The colorimetric display is suited for readouts using either human eyes or hand-held imaging devices such as smart phones. This work promises of a new strategy of developing high performance paper-based sensors for point-of-care diagnosis applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 238, January 2017, Pages 138-144
Journal: Sensors and Actuators B: Chemical - Volume 238, January 2017, Pages 138-144
نویسندگان
Guoying Dai, Jinglin Hu, Xueyan Zhao, Ping Wang,