کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7142849 | 1462055 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Visualization of G-quadruplexes in gel and in live cells by a near-infrared fluorescent probe
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Visualization of G-quadruplexes in gel and in live cells by a near-infrared fluorescent probe Visualization of G-quadruplexes in gel and in live cells by a near-infrared fluorescent probe](/preview/png/7142849.png)
چکیده انگلیسی
G-quadruplexes, one of the most significant secondary structure of nucleic acid, are formed by stacking G quartets, which received broad interests due to their involvement in telomere function, gene transcription and recombination. As for ligand, better selectivity for G-quadruplex against other DNA or RNA structures and higher ability to stabilize G-quadruplex are necessary. In addition, developing a new probe to recognize G-quadruplex is desired for utilizing G-quadruplex structure to relevant biological processes. In this study, we report a Cy5 labelled ligand (named PDP-Cy5) developed as a near-infrared fluorescent ligand for G-quadruplexes both in DNA and RNA. The results indicated that PDP-Cy5 selectively induced the formation of intramolecular G-quadruplexes with strong binding affinity. Furthermore, this Cy5 labelled ligand can effectively stabilize G-quadruplexes. Moreover, the direct visualization of G-quadruplexes in gel, even on cell level is realized by using PDP-Cy5.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 236, 29 November 2016, Pages 268-275
Journal: Sensors and Actuators B: Chemical - Volume 236, 29 November 2016, Pages 268-275
نویسندگان
Fan Wu, Chaoxing Liu, Yuqi Chen, Shixi Yang, Jiahui Xu, Rong Huang, Xiang Wang, Manjia Li, Wenting Liu, Wuxiang Mao, Xiang Zhou,