کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7143267 1462057 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Differential-mode HEMT-based biosensor for real-time and label-free detection of C-reactive protein
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Differential-mode HEMT-based biosensor for real-time and label-free detection of C-reactive protein
چکیده انگلیسی
In this paper, we have investigated the characteristics of the differential-mode high electron mobility transistor (HEMT)-based biosensor. An Au-gate HEMT is the sensing device that reacts with biomolecules, whereas the Pt-gate HEMT is the dummy device used in the differential-mode detection circuit. Both the biosensing HEMT (Bio-HEMT) and reference HEMT (Ref-HEMT) were biased by a Pt quasi-reference electrode (QRE). The Pt QRE was used to replace the bulky conventional reference electrode. The binding of 11-mercaptoundecanoic acid, CRP-antibody, and CRP caused a variation in Bio-HEMT gate surface potential, which was confirmed by X-ray photoelectron spectroscopy. Moreover, we measured the variation of output voltage with light intensity and temperature, which was eliminated as a common-mode signal by the differential-mode measurement technique. Therefore, this technique can improve the detection limit in low CRP concentration. The output voltage of the sensor system is nonlinearly proportional to the CRP concentration from 0.01 ng/mL to 1000 ng/mL. These results indicated that the Bio-HEMT/Ref-HEMT/QRE could be applied to a portable biomolecule detection system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 234, 29 October 2016, Pages 316-323
نویسندگان
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