کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7147170 | 1462098 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Colorimetric determination of DNA concentration and mismatches using hybridization-mediated growth of gold nanoparticle probes
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Colorimetric determination of DNA concentration and mismatches using hybridization-mediated growth of gold nanoparticle probes Colorimetric determination of DNA concentration and mismatches using hybridization-mediated growth of gold nanoparticle probes](/preview/png/7147170.png)
چکیده انگلیسی
A novel colorimetric approach to sense DNA samples was demonstrated successfully based on a concept of hybridization-mediated growth of gold nanoparticles probes (AuNP probes). The growth size and conformation of the probes are dominated by DNA samples that hybridize with the probes. The growth probes serve as nanoparticle biosensors that can be utilized to analyze concentrations of DNA samples or to discriminate mismatches of DNA samples to the probes (complementarity of DNA) in terms of size- and shape-dependence optical properties. By means of the proposed method, not only the semi-quantitation of target DNA but also the differentiation of DNA mismatches can be readily achieved with a naked eye or simple spectrophotometer. This method features small consumption of reagents, a satisfactory detection limit (about 60Â nM), a small duration of assay (a couple of minutes), and a simple procedure without thermal control.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 192, 1 March 2014, Pages 77-82
Journal: Sensors and Actuators B: Chemical - Volume 192, 1 March 2014, Pages 77-82
نویسندگان
Wei-Feng Fang, Wen-Jone Chen, Jing-Tang Yang,