کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7147489 | 1462100 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Amperometric biosensors for NADH based on hyperbranched dendritic ferrocene polymers and Pt nanoparticles
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
The electrocatalytic performance of electrodes modified with Pt nanoparticles (PtNPs) and two dendritic hyperbranched carbosilane polymers, polydiallylmethylsilane (PDAMS) and polymethyldiundecenylsilane (PMDUS) with interacting ferrocenes, has been investigated for the NADH oxidation. The catalytic synergy of PtNPs with the interacting ferrocenes is discussed in relation with the polymer structure. This effect have allowed us to develop efficient biosensors capable of measuring NADH from +0.3 V (vs. SCE) providing a total protection vs. the poisoning of the electrodes. The polymer/PtNPs/Pt electrodes tolerate wide linear concentration ranges for NADH to 2.5 mM (R = 0.9979) and 2.1 mM (R = 0.99849), with detection limits of 4.78 μM and 6.18 μM and sensitivities of 68.24 and 40.21 μA mMâ1 cmâ2 for the PDAMS/PtNPs/Pt and PMDUS/PtNPs/Pt respectively. In the light of the good results obtained, novel amperometric alcohol biosensors were also successfully prepared with alcohol dehydrogenase (ADH). These devices showed more affinity for methanol than for ethanol, with a wide linear range to 30 mM and sensitivities of 0.957 and 0.756 μA mMâ1 cmâ2 for the ADH/PDAMS/PtNPs/Pt and ADH/PMDUS/PtNPs/Pt respectively. The oxidation potential of the NADH enzymatically produced was negatively shifted to +0.25 V.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 190, January 2014, Pages 111-119
Journal: Sensors and Actuators B: Chemical - Volume 190, January 2014, Pages 111-119
نویسندگان
Almudena Jiménez, M. Pilar GarcÃa Armada, José Losada, Carlos Villena, Beatriz Alonso, Carmen M. Casado,