کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7147691 | 1462099 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of metolcarb binding to DNA by spectroscopic and chemometrics methods with the use of acridine orange as a probe
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Determination of metolcarb binding to DNA by spectroscopic and chemometrics methods with the use of acridine orange as a probe Determination of metolcarb binding to DNA by spectroscopic and chemometrics methods with the use of acridine orange as a probe](/preview/png/7147691.png)
چکیده انگلیسی
The interaction of metolcarb (MTMC), an agricultural pesticide, with calf thymus DNA (ctDNA) under simulative physiological buffer (pH 7.4) was determined with the use of acridine orange (AO) as a spectral probe by spectroscopic methods including UV-vis absorption, fluorescence, circular dichroism (CD), and Fourier transform infrared (FT-IR) spectroscopy along with viscosity measurement and molecular docking techniques. A chemometrics approach, multivariate curve resolution-alternating least squares (MCR-ALS), was applied to resolve the combined UV-vis absorption and fluorescence spectroscopic data matrix. The concentration profiles of the three reaction components (MTMC, ctDNA, and MTMC-ctDNA complex) and their pure spectra were then successfully obtained to evaluate the progress of MTMC interacting with ctDNA. It was found that MTMC molecule could interact with ctDNA through an intercalative mode as evidenced by the competitive binding with intercalator AO, decrease in single-stranded ctDNA quenching effect, increases in ctDNA viscosity, and CD spectral changes. The FT-IR analysis indicated that MTMC was more prone to bind to the G-C bases rich region of ctDNA. Furthermore, the molecular docking study was used to confirm and illustrate the specific binding.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 191, February 2014, Pages 464-472
Journal: Sensors and Actuators B: Chemical - Volume 191, February 2014, Pages 464-472
نویسندگان
Yu Li, Guowen Zhang, Junhui Pan, Yue Zhang,