کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7147877 | 1462099 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Direct electron transfer and electrocatalytic properties of immobilized hemoglobin onto glassy carbon electrode modified with ionic-liquid/titanium-nitride nanoparticles: Application to nitrite detection
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Glassy carbon (GC) electrode modified with covalently attached amine-terminated ionic liquid (NH2-IL) and titanium nitrite nanoparticles (TiNnp) was used as support for immobilization of hemoglobin (Hb) protein. The presence of well-defined and nearly reversible CV peaks with E°Ⲡof â0.03 V (vs. Ag/AgCl) indicated the successful immobilization of Hb on GC/TiNnp/NH2-IL surface. The surface coverage of Hb immobilized on nanocomposite film (Î), and heterogeneous electron transfer rate constant (ks) were 5.12 (±0.05) Ã 10â11 mol cmâ2 and 5.10 ± 0.10 sâ1 respectively, indicating high loading capacity of nanocomposite for direct electron transfer of Hb on the electrode surface. Moreover, the GC/TiNnp/NH2-IL/Hb electrode revealed excellent electrocatalytic activity toward nitrite reduction. This sensor exhibited fast amperometric response with sensitivity of 0.027 ± 0.009 μA/μM and limit of detection of 0.10 ± 0.010 μM at the concentration range up to 2 mM. The calculated Michaelis-Menten constant (KM) of 0.065 (±0.030) mM indicated that Hb immobilized onto the nanocomposite film retained its native activity. The sensor also showed good stability, reproducibility and long lifetime and it was successfully applied for nitrite detection in real samples with obtained satisfactory results.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 191, February 2014, Pages 625-633
Journal: Sensors and Actuators B: Chemical - Volume 191, February 2014, Pages 625-633
نویسندگان
Shaghayegh Saadati, Abdollah Salimi, Rahman Hallaj, Amin Rostami,