کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7195956 | 1468281 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Conductive composites for oligonucleotide detection
ترجمه فارسی عنوان
کامپوزیت های هدایت کننده برای تشخیص الیگونوکلئوتید
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A new method for oligonucleotide detection is presented based on oligonucleotide cross-linked polymer composites. Conductive carbon nanoparticles are incorporated into a DNA-functionalised polymer, containing partially complementary oligonucleotide cross-linkers, which is polymerised in situ upon interdigitated electrodes. In the presence of an aqueous solution of a specific analyte oligonucleotide sequence, the cross-linkers are cleaved, leading to increased swelling. As the polymer swells the relative density of the conductive particles decreases, leading to an easily measurable decrease in electrical conductivity. We demonstrate that such are capable of discriminating between analyte and control solutions, with single-base specificity, in under 3Â min. The lower detection limit of these composites is of the order of 10Â nM. The swelling characteristics of these composites is confirmed by optical imaging and the effects of varying temperature upon such composites are also reported.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensing and Bio-Sensing Research - Volume 17, February 2018, Pages 1-6
Journal: Sensing and Bio-Sensing Research - Volume 17, February 2018, Pages 1-6
نویسندگان
David C. Ferrier, Michael P. Shaver, Philip J.W. Hands,