Cell elasticity with altered cytoskeletal architectures across multiple cell types

- Elastic moduli are measured by AFM on five cell lines subject to cytoskeletal drugs.
- Control of indentation depth and approach velocity maintains the probing frequency.
- The contribution of F-actin and microtubules toward cell elasticity is probed.
- F-actin contributes more to elastic stiffness than microtubules in non-cancer cells.
- Destabilizing microtubule dynamics in cancer cells results in increased stiffness.