Effective PEI-mediated delivery of CRISPR-Cas9 complex for targeted gene therapy

Schematic of gene delivery by cationic polymer polyethylenimine (PEI) - branched PEI 25 kDa (BPEI-25K). The plasmid expressing both CRISPR/Cas9 and guide RNA forms compact complexes with BPEI-25K and is protected from degradation by lysosomal enzymes. The plasmid DNA is released into the cytosol and enters into the nucleus. The plasmid is translated into Cas9:sgRNA complex and nuclear localized for gene editing.123