Rapid multiplexed detection of beta-amyloid and total-tau as biomarkers for Alzheimer's disease in cerebrospinal fluid

Multiplexed detection of biomarkers, beta-amyloid (Aβ42) and total tau (T-tau), in both buffer and cerebrospinal fluid (CSF) for Alzheimer's disease (AD) using label-free optical nanosensors has been demonstrated. 7.8 pg/ml of Aβ42 in buffer and 15.6 pg/ml of T-au in buffer can be readily detected with very good specificity. Aβ42 and T-tau spiked in CSF have been detected successfully, indicating the feasibility of the nanosensors to detect these biomarkers in clinical samples.168