کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7238692 | 1471145 | 2018 | 34 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Toward detection of toxoplasmosis from urine in mice using hydro-gel nanoparticles concentration and parallel reaction monitoring mass spectrometry
ترجمه فارسی عنوان
جهت تشخیص توکسوپلاسموز از ادرار در موشها با استفاده از غلظت نانوذرات هیدروژل و طیف سنجی جرمی واکنش موازی
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
سایر رشته های مهندسی
مهندسی پزشکی
چکیده انگلیسی
Diagnosis of clinical toxoplasmosis remains a challenge, thus limiting the availability of human clinical samples. Though murine models are an approximation of human response, their definitive infection status and tissue availability make them critical to the diagnostic development process. Hydrogel mesh nanoparticles were used to concentrate antigen to detectable levels for mass spectrometry. Seven T. gondii isolates were used to develop a panel of potential peptide sequences for detection by parallel reaction monitoring (PRM) mass spectrometry. Nanoparticles were incubated with decreasing concentrations of tachyzoite lysate to explore the limits of detection of PRM. Mice whose toxoplasmosis infection status was confirmed by quantitative real-time PCR had urine tested by PRM after hydrogel mesh concentration for known T. gondii peptides. Peptides from GRA1, GRA12, ROP4, ROP5, SAG1, and SAG2A proteins were detected by PRM after nanoparticle concentration of urine, confirming detection of T. gondii antigen in the urine of an infected mouse.133
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nanomedicine: Nanotechnology, Biology and Medicine - Volume 14, Issue 2, February 2018, Pages 461-469
Journal: Nanomedicine: Nanotechnology, Biology and Medicine - Volume 14, Issue 2, February 2018, Pages 461-469
نویسندگان
Hannah E. MSPH, Paul PhD, Noelia DVM, Raúl BS, Cristina BS, Andrea BS, Cusi DVM, Francesca DVM, Edgar BS, Juan PhD, Maritza PhD, Vern B. PhD, Robert H. MD, Lance MD/PhD, Alessandra PhD,