Synergistic chemo-photodynamic therapy by “big & small combo nanoparticles” sequential release system

Here we propose a “big & small combo nanoparticles (NPbig&small)” system for double loading PSs methylene blue (MB) and single absorbing chemotherapeutics drug Gemcitabine hydrochloride (GM·HCl). The “grown-in” MB from NPbig&small show two-peak sequential release profile, significantly improve the absorbed chemo therapeutic efficacy of GM·HCl. Thus, NPbig&small chemo-photodynamic system and the programmable sequential release mechanism provide a promising platform that ensures an enhanced synergistic chemo-photodynamic effect in cancer treatment.295