کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
741323 | 1462061 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Conversion of protein net charge via chemical modification for highly sensitive prion detection using field effect transistor (FET) biosensor
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Conversion of protein net charge via chemical modification for highly sensitive prion detection using field effect transistor (FET) biosensor Conversion of protein net charge via chemical modification for highly sensitive prion detection using field effect transistor (FET) biosensor](/preview/png/741323.png)
چکیده انگلیسی
A robust and applicable strategy of signal amplification for field effect transistor (FET) biosensor is of scientifically and technologically importance to detect wide range of target molecules, particularly for the molecules of low charge density, and to configure highly sensitive analysis. In this communication, we demonstrate a simple technique to modify the net charge of target protein based on the concept of protein conjugation for the detection of prion, which is infectious and transmissible protein in neurodegenerative disorders. The increases of prion net charge after chemical modification generate signal amplification and have remarkably enhanced the sensitivity of FET biosensor below than femtomolar (fM) level. Thus, we suggest that such strategy is promising technique for achieving a highly sensitive detection at very low concentration that is useful for early determination of protein biomarkers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 230, July 2016, Pages 374-379
Journal: Sensors and Actuators B: Chemical - Volume 230, July 2016, Pages 374-379
نویسندگان
Shofarul Wustoni, Sho Hideshima, Shigeki Kuroiwa, Takuya Nakanishi, Yasuro Mori, Tetsuya Osaka,