کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
746305 894450 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Detection of pneumolysin in human urine using an immunosensor on screen-printed carbon electrodes
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Detection of pneumolysin in human urine using an immunosensor on screen-printed carbon electrodes
چکیده انگلیسی

A voltammetric immunosensor for pneumolysin (PLY), based on the electrochemical detection of indigo blue, produced by alkaline phosphatase (AP) when 3-indoxyl phosphate (3-IP) is used as enzymatic substrate, is prepared and evaluated for measuring this toxin in human urine samples.The single-use immunosensor is fabricated by deposition of biotinylated anti-PLY monoclonal antibodies onto pre-oxidised streptavidin coated screen-printed carbon electrodes (SPCEs). Rabbit polyclonal IgGs anti-PLY are used in combination with an anti-rabbit IgG alkaline phosphatase conjugate as detection antibodies.The estimated limit of detection of the purified recombinant toxin is 0.05 ng/ml in Tris-buffer and 0.12 ng/ml in undiluted urine samples, but the same linear range, between 0.25 and 5.0 ng/ml, is obtained in both cases. The immunosensor is also applied to detect pneumolysin in urine samples from two pneumococcal pneumonia patients.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 113, Issue 2, 27 February 2006, Pages 1005–1011
نویسندگان
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