کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
746546 894461 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
One-step synthesis of silver nanoparticles/carbon nanotubes/chitosan film and its application in glucose biosensor
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
One-step synthesis of silver nanoparticles/carbon nanotubes/chitosan film and its application in glucose biosensor
چکیده انگلیسی

One-step synthesis of silver nanoparticles/carbon nanotubes/chitosan film (Ag/CNT/Ch) was firstly proposed as a novel immobilization matrix for the enzymes to fabricate sensitive glucose biosensor. The biosensor was prepared by embedding horseradish peroxidase (HRP) and glucose oxidase (GOD) in Ag/CNT/Ch hybrid films based on layer by layer technique. The electron mediator, o-phenylenediamine (OPD), was also co-immobilized with HRP in the inner layer on the surface of an indium tin oxide (ITO) electrode. Hydrogen peroxide produced by the GOD catalytic oxidation of glucose was then detected by OPD in the presence of HRP. Under the optimized experimental conditions, the resulting biosensor could detect glucose in a linear range from 0.5 to 50 μM with a detection limit of 0.1 μM at a signal-to-noise ratio of 3. The sensitivity was calculated as 135.9 μA mM−1. The proposed biosensor offered sensitive amperometric responses to glucose based on the Ag/CNT/Ch films.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 137, Issue 2, 2 April 2009, Pages 768–773
نویسندگان
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