کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7583857 | 1492022 | 2019 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ultrasensitive and eco-friendly immunoassays based monoclonal antibody for detection of deoxynivalenol in cereal and feed samples
ترجمه فارسی عنوان
آنتیبادی مونوکلونال مبتنی بر ایمونواسکشن های حساس و سازگار با محیط زیست برای تشخیص دکسینوالنول در نمونه های غلات و خوراکی
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Ultrasensitive immunoassays, including an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a lateral-flow immunochromatographic assay (ICA), were developed based on a monoclonal antibody for the analysis of deoxynivalenol in food and feed samples. With 0.01â¯M PBS, 20% ethanol-PBS, and 60% ethanol-PBS extraction, which are environmentally safe, the 50% inhibitory concentration (IC50) and limit of detection (LOD) values were 1.83-4.68â¯Î¼g/kg and 0.241-0.664â¯Î¼g/kg, respectively, with recovery rates of 87.7%-137% and coefficient variation values of 3.99-9.88% (intra-assay) and 4.17-9.81% (inter-assay) for the ic-ELISA relative to the results obtained by liquid chromatography-tandem mass spectrometry (LC-MS). For the ICA strip, the visual LODs were 10-150â¯Î¼g/kg, the cut-off values were 50-750â¯Î¼g/kg, and the calculated LODs were 1.97-46.8â¯Î¼g/kg, with different sample extraction solutions, and the recovery rates were 66.7%-127%. These methods are sensitive, simple and safe, providing an auxiliary analytical tool for screening the massive samples in markets.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 270, 1 January 2019, Pages 130-137
Journal: Food Chemistry - Volume 270, 1 January 2019, Pages 130-137
نویسندگان
Dezhao Kong, Xiaoling Wu, Yue Li, Liqiang Liu, Shanshan Song, Qiankun Zheng, Hua Kuang, Chuanlai Xu,