کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7583930 | 1492021 | 2019 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A competitive immunoassay for biotin detection using magnetic beads and gold nanoparticle probes
ترجمه فارسی عنوان
یک آزمایش ایمنی رقابتی برای تشخیص بیوتین با استفاده از دانه های مغناطیسی و پروب نانوذرات طلا
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کلمات کلیدی
ایمنی رقابتی، تشخیص بیوتین، دانه های مغناطیسی، نانوذرات طلا،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Haptens are small molecules with low molecular weight that include biotin and many toxins in food. In this study, we used biotin as a model molecule for hapten detection. In this competitive immunoassay anti-biotin antibody-modified magnetic beads (Ab-MBs) and biotinylated thiol-DNA gold nanoparticles (biotin-GNPs) were used. The assay contains three reactions, the mixing of the sample and Ab-MBs, the capture of biotin-GNPs by Ab-MBs and the magnetic attraction. When biotin molecules were absent, the solution was transparent because biotin-GNPs bound to Ab-MBs which were caught by an external magnetic field. When biotin was present, the supernatant was red because the Ab-MBs bound to the analytes and the gold nanoparticles were still in solution. It was possible to complete all the operating steps in 15â¯min. The limit of detection (LOD) was 2â¯pmol. This rapid competitive-immunoassay has potential for application in detection of other haptens.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 271, 15 January 2019, Pages 440-444
Journal: Food Chemistry - Volume 271, 15 January 2019, Pages 440-444
نویسندگان
Wen-Zhi Lin, Yi-Hsiang Chen, Chih-Kai Liang, Cheng-Che Liu, Shao-Yi Hou,