کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7584051 | 1492022 | 2019 | 23 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fluorescence strategy for sensitive detection of adenosine triphosphate in terms of evaluating meat freshness
ترجمه فارسی عنوان
استراتژی فلورسانس برای شناسایی حساس آدنوزین تری فسفات از نظر ارزیابی طراوت گوشت
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A novel simple, sensitive and reliable sensor based on S1 nuclease, FAM-labeled ssDNA (DNA-F) and graphene oxide (GO) was developed for detecting adenosine triphosphate (ATP) and evaluating the freshness of meat (beef) samples. With S1 nuclease as the cleaver of DNA-F and ATP as the inhibitor of S1 nuclease, the fluorescence of DNA-F could be obviously quenched by GO, which exhibits the fluorescence of system gradually decrease as the increasing ATP concentration. Under the optimal conditions, a linear correlation between the fluorescence and the ATP concentration from 20â¯Î¼M to 3500â¯Î¼M is obtained with a detection limit of 3.2â¯Î¼M. Furthermore, the proposed ATP detection method was applied to the ATP detection in microorganisms in meat samples, which acquired the satisfying results, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 270, 1 January 2019, Pages 573-578
Journal: Food Chemistry - Volume 270, 1 January 2019, Pages 573-578
نویسندگان
Zhenning Liu, Yinghui Zhong, Yue Hu, Lei Yuan, Rui Luo, Dan Chen, Miao Wu, Hui Huang, Yongxin Li,