کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7584417 | 1492026 | 2018 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Full inhibition of Whangkeumbae pear polyphenol oxidase enzymatic browning reaction by l-cysteine
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
The enzymatic browning reaction caused by polyphenol oxidase (PPO) has a negative effect on the processing of fruits and vegetables. However, some chemical inhibitors have been used to prevent enzymatic browning reaction, although they may be toxic and potentially hazardous to use in food. In this study, PPO was isolated and purified from the Whangkeumbae pear, and four food grade inhibitors were used to prevent the enzymatic browning reaction. The results showed that the PPO activity increased by 32.93-fold; its yield was 0.2â¯U/100â¯U, and the specific activity was 519,895.73â¯U/mg protein. The molecular weight of the PPO was approximately 44â¯kDa. The most potent inhibitor was l-cysteine, which fully inhibited the PPO activity at a concentration of 0.8â¯mg/mL. The type of inhibition of l-cysteine was noncompetitive. It suggests that l-cysteine can be utilized to prevent enzymatic browning reaction during the processing of pear juice.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 266, 15 November 2018, Pages 1-8
Journal: Food Chemistry - Volume 266, 15 November 2018, Pages 1-8
نویسندگان
Xiran Zhou, Yanju Xiao, Xianghong Meng, Bingjie Liu,