کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7584803 | 1492030 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier](/preview/png/7584803.png)
چکیده انگلیسی
Immunochromatographic assays (ICAs) are most frequently used for on-site rapid screening of clenbuterol. To improve sensitivity, a novel probe with bacteria as signal carriers was developed. Bacteria can load a great deal of gold nanoparticles (AuNPs) on their surface, meaning much fewer antibodies are needed to produce clearly visible results, although low concentrations of antibody could also trigger fierce competition between free analyte and the immobilized antigen. Thus, a limited number of antibodies was key to significantly improved sensitivity. Analytical conditions, including bacterial species, coupling method, and concentration, were optimized. The visual detection limit (VDL) for clenbuterol was 0.1â¯ng/mL, a 20-fold improvement in sensitivity compared with traditional strips. This work has opened up a new route for signal amplification and improved performance of ICAs. Furthermore, inactivated bacteria could also be environment-friendly and robust signal carriers for other biosensors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 262, 1 October 2018, Pages 48-55
Journal: Food Chemistry - Volume 262, 1 October 2018, Pages 48-55
نویسندگان
Qiong Huang, Tong Bu, Wentao Zhang, Lingzhi Yan, Mengyue Zhang, Qingfeng Yang, Lunjie Huang, Baowei Yang, Na Hu, Yourui Suo, Jianlong Wang, Daohong Zhang,