کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7584957 | 1492031 | 2018 | 25 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Absolute quantification of two antimicrobial peptides αS2-casein182-207 and αS2-casein151-181 in bovine milk by UHPLC-ESI-MS/MS in sMRM mode
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
To evaluate if the recently identified antimicrobial milk peptides αS2-casein182-207 and αS2-casein151-181 can be of physiological or technological relevance, their presence in commercial milk was investigated. Thus, a UHPLC-ESI-MS/MS scheduled multiple reaction monitoring method was developed for quantification using stable isotope-labeled peptides. The developed protocol gave very good linear response (R2â¯>â¯0.99) for both peptides, recovery >95% and coefficients of variations of 5.51% and 4.79%. The limits of detection (0.3â¯nM αS2-casein182-207 and 0.5â¯nM αS2-casein151-181) were low enough to detect both peptides without further enrichment. The highest peptide concentration was recorded in pasteurized milk (1.36â¯Î¼M αS2-casein182-207 and 0.20â¯Î¼M αS2-casein151-181). The concentration of αS2-casein182-207 was in a range that was previously reported to act bacteriostatic against Bacillus subtilis. Industrial milk processing led to a significant loss of both peptides resulting in a mean concentration of 0.47â¯ÂµM αS2-casein182-207 and 0.098â¯ÂµM αS2-casein151-181 in ultrahigh-temperature milk.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 261, 30 September 2018, Pages 15-20
Journal: Food Chemistry - Volume 261, 30 September 2018, Pages 15-20
نویسندگان
Yufang Liu, Monika Pischetsrieder,