کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7585087 | 1492033 | 2018 | 35 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lipase-catalysed esters synthesis of cafestol and kahweol
ترجمه فارسی عنوان
سنتز کیست های لیپاز کاتالیز شده کافئین و کایوئول
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Cafestol and kahweol (C&K), two coffee diterpene alcohols with structural similarity which exhibit anticarcinogenic effects, were isolated from green coffee Arabica beans, followed by their lipase-catalysed esterification and purification by preparative high-performance liquid chromatography (HPLC). The isolation and enzymatic synthesis parameters of C&K esters were studied, with the latter optimised by a Central Composite Design; both procedures were monitored by gas chromatography. Scale up and improved isolation conditions resulted in 1.29â¯g of C&K, with 98% purity from 300â¯g of green Arabica beans. The highest C&K ester yields were observed using an alcohol:fatty acid molar ratio of 1:5, 73.3â¯mgâ¯mLâ1 of CAL-B enzyme, 70â¯Â°C and 240â¯rpm for 3â¯days in toluene, leading to 85-88% conversion among a variety of tested C&K esters, including n-C14:0-C20:0, C18:1, C18:2 and C18:3.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 259, 1 September 2018, Pages 226-233
Journal: Food Chemistry - Volume 259, 1 September 2018, Pages 226-233
نویسندگان
Fábio Junior Moreira Novaes, Ivaldo Itabaiana Junior, Felipe Korbus Sutili, Philip John Marriott, Humberto Ribeiro Bizzo, Francisco Radler de Aquino Neto, Rodrigo Octávio Mendonça Alves de Souza, Claudia Moraes Rezende,