کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7585573 | 1492037 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fluoroimmunoassays for the detection of zearalenone in maize using CdTe/CdS/ZnS quantum dots
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
CdTe/CdS/ZnS quantum dots (QDs) were synthesized in aqueous phase and conjugated with a new anti-zearalenone (ZEN) monoclonal antibody. Using this novel fluorescent probe, a fluoroimmunoassay (FLISA) and a rapid immunochromatographic strip (ICTS) were developed for the detection of ZEN in maize. Our proposed FLISA allowed for ZEN determination in the range of 0.038-0.977â¯ng/mL with an IC50 of 0.162â¯ng/mL and a limit of detection (LOD) of 0.012â¯ng/mL occurring in maize. The rapid ICTS had a visual LOD of 1.0â¯ng/mL in buffer solution and 1.5â¯ng/mL in maize extract. These two QDs-based immunoassays were all successfully verified by commercial ELISA kits. The results confirmed that: firstly, the FLISA can be used as a robust method for the sensitive detection of ZEN; and secondly, the ICTS is ideally suited for rapidly screening large numbers of samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 255, 30 July 2018, Pages 421-428
Journal: Food Chemistry - Volume 255, 30 July 2018, Pages 421-428
نویسندگان
Fuyuan Zhang, Bing Liu, Wei Sheng, Yan Zhang, Qi Liu, Shijie Li, Shuo Wang,