کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7586950 1492075 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of molecular imprinted column-on line-two dimensional liquid chromatography for selective determination of clenbuterol residues in biological samples
ترجمه فارسی عنوان
توسعه کروماتوگرافی مایع کروماتوگرافی خط دو بعدی برای تعیین انتخاب بقایای کلنبوترول در نمونه های بیولوژیکی
کلمات کلیدی
کلنبوترول، ستون های یکپارچه وارد شده مولکولی، کروماتوگرافی مایع دو بعدی، کبد خوک، ادرار خوک،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A novel method coupling molecular imprinted monolithic column with two-dimensional liquid chromatography was developed and validated for the analysis of clenbuterol in pork liver and swine urine samples. The polymers were characterized by using Fourier transform infrared spectroscopy, nitrogen adsorption desorption analyses, frontal analysis and the adsorption of selectivity. The results indicated that the imprinted columns were well prepared and possessed high selectivity adsorption capacity. Subsequently, the MIMC-2D-LC (molecular imprinted monolithic column-two dimensional liquid chromatography) method was developed for the selective analysis of clenbuterol in practical samples. The accuracy ranged from 94.3% to 99.7% and from 93.7% to 99.6% for liver and urine, respectively. The relative standard deviation (RSD) of repeatability was lower than 8.6% for both analyses. The limit of detections was 16 ng·mL−1 for liver and 25 ng·mL−1 for urine, respectively. Compared with the reported methods, the disturbance of endogenous impurity could be avoided by the 2D-LC method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 217, 15 February 2017, Pages 628-636
نویسندگان
, , , , , , , , ,