کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7587300 | 1492078 | 2017 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
NGS tools for traceability in candies as high processed food products: Ion Torrent PGM versus conventional PCR-cloning
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: NGS tools for traceability in candies as high processed food products: Ion Torrent PGM versus conventional PCR-cloning NGS tools for traceability in candies as high processed food products: Ion Torrent PGM versus conventional PCR-cloning](/preview/png/7587300.png)
چکیده انگلیسی
The Next Generation Sequencing methodologies are considered the next step within DNA-based methods and their applicability in different fields is being evaluated. Here, we tested the usefulness of the Ion Torrent Personal Genome Machine (PGM) in food traceability analyzing candies as a model of high processed foods, and compared the results with those obtained by PCR-cloning-sequencing (PCR-CS). The majority of samples exhibited consistency between methodologies, yielding more information and species per product from the PGM platform than PCR-CS. Significantly higher AT-content in sequences of the same species was also obtained from PGM. This together with some taxonomical discrepancies between methodologies suggest that the PGM platform is still pre-mature for its use in food traceability of complex highly processed products. It could be a good option for analysis of less complex food, saving time and cost per sample.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 214, 1 January 2017, Pages 631-636
Journal: Food Chemistry - Volume 214, 1 January 2017, Pages 631-636
نویسندگان
Marta Muñoz-Colmenero, Jose Luis MartÃnez, AgustÃn Roca, Eva Garcia-Vazquez,