کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7594598 | 1492120 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enzymatic production of bioactive docosahexaenoic acid phenolic ester
ترجمه فارسی عنوان
تولید آنزیمی از اسید فنولیک اسید داکتیکاکسانوئیک زیست فعال
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کلمات کلیدی
DPPHBHTABTSAβ2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) - 2،2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)amyloid-β - آمیلوئید βdocosahexaenoic acid - اسید داکوزاگزوائونیکDHA - دوکوساهگزائنوئیک اسیدbutylated hydroxytoluene - هیدروکسی تورولین باتلاقیCalb - کالباسCandida antarctica lipase B - کاندیدا آنتارکتازی لیپاز B
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Docosahexaenoic acid (DHA) is increasingly considered for its health benefits. However, its use as functional food ingredient is still limited by its instability. In this work, we developed an efficient and solvent-free bioprocess for the synthesis of a phenolic ester of DHA. A fed-batch process catalyzed by Candida antarctica lipase B was optimised, leading to the production of 440 g/L vanillyl ester (DHA-VE). Structural characterisation of the purified product indicated acylation of the primary OH group of vanillyl alcohol. DHA-VE exhibited a high radical scavenging activity in acellular systems. In vivo experiments showed increased DHA levels in erythrocytes and brain tissues of mice fed DHA-VE-supplemented diet. Moreover, in vitro neuroprotective properties of DHA-VE were demonstrated in rat primary neurons exposed to amyloid-β oligomers. In conclusion, DHA-VE synergized the main beneficial effects of two common natural biomolecules and therefore appears a promising functional ingredient for food applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 171, 15 March 2015, Pages 397-404
Journal: Food Chemistry - Volume 171, 15 March 2015, Pages 397-404
نویسندگان
Mohamed H. Roby, Ahmad Allouche, Layal Dahdou, Vanessa C. De Castro, Paulo H. Alves da Silva, Brenda N. Targino, Marion Huguet, Cédric Paris, Françoise Chrétien, Rosa-Maria Guéant, Stéphane Desobry, Thierry Oster, Catherine Humeau,