کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7596997 | 1492128 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Smart phone: A popular device supports amylase activity assay in fisheries research
ترجمه فارسی عنوان
گوشی هوشمند: یک دستگاه محبوب از آزمون فعالیت آمیلاز در تحقیقات ماهیگیری استفاده می کند
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کلمات کلیدی
فعالیت آمیلاز، گوشی های هوشمند، تجزیه و تحلیل تصویر دیجیتال، تحقیقات شیلات،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Colourimetric determinations of amylase activity were developed based on a standard dinitrosalicylic acid (DNS) staining method, using maltose as the analyte. Intensities and absorbances of red, green and blue (RGB) were obtained with iPhone imaging and Adobe Photoshop image analysis. Correlation of green and analyte concentrations was highly significant, and the accuracy of the developed method was excellent in analytical performance. The common iPhone has sufficient imaging ability for accurate quantification of maltose concentrations. Detection limits, sensitivity and linearity were comparable to a spectrophotometric method, but provided better inter-day precision. In quantifying amylase specific activity from a commercial source (PÂ >Â 0.02) and fish samples (PÂ >Â 0.05), differences compared with spectrophotometric measurements were not significant. We have demonstrated that iPhone imaging with image analysis in Adobe Photoshop has potential for field and laboratory studies of amylase.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 163, 15 November 2014, Pages 87-91
Journal: Food Chemistry - Volume 163, 15 November 2014, Pages 87-91
نویسندگان
Karun Thongprajukaew, Aree Choodum, Barunee Sa-E, Ummah Hayee,