کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7599083 | 1492142 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Probing the micellar solubilisation and inter-micellar exchange of polyphenols using the DPPH free radical
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Encapsulation of polyphenols can be used for improving their stability and targeting. We present here a spectrophotometric method to probe the micellar solubilisation and inter-micellar exchange of polyphenols using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical as a visible probe. Our method relies on the partitioning of DPPH into micelles, on the reduction of DPPH by polyphenols, and on the change in absorbance of DPPH when reduced/oxidised. Hence, an absorbance drop at 528Â nm gives evidence of the co-localisation of polyphenols and DPPH in micelles. Using catechin and sodium dodecyl sulfate (SDS) as model molecules, we have shown that the reduction stoichiometry increases up to the critical micelle concentration (CMC) of SDS, where it reaches a plateau: this is due to the solubilisation of catechin in pre-micellar aggregates and then in micelles. The initial rate of reduction increases with increasing SDS concentration up to the CMC and then decreases due to a dilution effect.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 149, 15 April 2014, Pages 114-120
Journal: Food Chemistry - Volume 149, 15 April 2014, Pages 114-120
نویسندگان
Mickaël Laguerre, Virginie Hugouvieux, Nükhet Cavusoglu, Fabien Aubert, Aurélie Lafuma, Hélène Fulcrand, Céline Poncet-Legrand,