Structural analysis of linear mixed-linkage glucooligosaccharides by tandem mass spectrometry

► Mass spectrometry in structure analysis of mixed-linkage glucooligosaccharides. ► Emphasis is on mixed-linkage glucooligosaccharides that contain (1 → 3) linkages. ► The disadvantages of using positive mode mass spectrometry are demonstrated. ► Negative mode data is preferable for structure analysis of glucooligosaccharides.