Development and validation of an HPLC method for the determination of six penicillin and three amphenicol antibiotics in gilthead seabream (Sparus Aurata) tissue according to the European Union Decision 2002/657/EC

► A multi-residue HPLC-DAD method for the determination of amphenicols and penicillins was developed. ► An optimised SPE was applied for the isolation of amphenicols and penicillins from fish. ► Method validation was performed according to the European Union decision 2002/657/EC. ► The method was applied in gilthead seabream from aquaculture.