Effect of origin, breeding and processing conditions on the isotope ratios of bioelements in dry-cured ham

► IRMS was applied to six different types of dry-cured hams, with the aim of tracing their origin and production system. ► The stable isotope ratios of the main bioelements of three ham fractions (overall 11 ratios) were assessed on 86 samples. ► Pig origin had a significant effect on the isotope ratio variability of ham tissues. ► Pig genotype, feeding regime, ham seasoning time may generate differences in isotope ratios.