کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7641421 | 1494868 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A sensitive atomic absorption spectrometric metalloimmunoassay with copper nanoparticles labeling
ترجمه فارسی عنوان
متالویمونوسیت طیف سنج جذب اتمی با برچسب نانوذرات مس
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A sensitive metalloimmunoassay based on copper nanoparticle labeling was demonstrated with atomic absorption spectrometry. Stable nanosized CuO particles of 5-10 nm diameter were synthesized by an alcothermal method, and conjugated to antibody under moderate conditions. The immunoassay conditions were optimized. After immunoreaction, the copper atoms were released with diluted nitric acid and measured by electro-thermal atomic absorption spectrometry. The atomic absorbance signal had a good linearity with logarithm of the CIgG (concentration of IgG, g/mL). The detection limit was calculated to be 0.19 ng/mL and the assay was linear in the range of 10â 9-10â 5 g/mL human IgG. The relative standard deviation (RSD) for 10 ng/mL human IgG was 3.0%. The human IgG results obtained by the proposed method correlated well with those obtained by inductively coupled plasma-based immunoassay.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microchemical Journal - Volume 126, May 2016, Pages 1-6
Journal: Microchemical Journal - Volume 126, May 2016, Pages 1-6
نویسندگان
Yuming Xu, Ying Gao, Xin Zhao, Xuemei Xu, Weiwei Zhou, Yu Liu, Chongying Li, Rui Liu,