The investigation of the interaction between Tropicamide and bovine serum albumin by spectroscopic methods

- The interaction of bovine serum albumin (BSA) and Tropicamide (TA) was studied.
- The fluorescence quenching mechanism is static quenching.
- The binding constants and binding sites were calculated.
- Hydrogen binds and vander Waals interaction force played a major role in stabilizing the complex.
- The TA affects the conformation of BSA.