Chiral derivatization coupled with liquid chromatography/mass spectrometry for determining ketone metabolites of hydroxybutyrate enantiomers

With PMP chiral derivatization, the D/L-2HB and D/L-3HB enantiomers can be distinctly determined by reversed-phase chromatographic separation. In addition, the detection sensitivities were greatly enhanced by LC-ESI-MS analysis due to the introduction of easily ionizable tertiary amino group from PMP.