کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7705101 1496911 2015 20 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrochemical oligonucleotide-based biosensor for the determination of lead ion
ترجمه فارسی عنوان
تعیین یون سرب از طریق بیوسنسور مبتنی بر الیگونوکلئوتید الکتروشیمیایی است
موضوعات مرتبط
مهندسی و علوم پایه شیمی الکتروشیمی
چکیده انگلیسی
The possibility of utilization of gold electrodes modified with short guanine-rich ssDNA probes for determination of Pb2 + was examined. Interaction between guanine residues and lead ion followed by formation of G-quadruplex structures was confirmed by electrochemical impedance spectroscopy investigations. An external cationic redox label, methylene blue, was employed in voltammetric measurements for analytical signal generation. It was shown that due to the G-quadruplex formation, the oligonucleotides in the recognition layer fold, which enhances the electron transfer between methylene blue and the electrode surface. The MB current signal rises proportionally to the lead ion concentration in the range from 0.05 to 1 μmol/L. The developed biosensor demonstrated high selectivity towards Pb2 + ion, with only minor response towards interfering metal cations. The calculated limit of detection was of 34.7 nmol/L. The utilization of the biosensor for Pb2 + determination in real samples of water was also tested.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioelectrochemistry - Volume 101, February 2015, Pages 35-41
نویسندگان
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