کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7705180 | 1496911 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
MWCNT-cysteamine-Nafion modified gold electrode based on myoglobin for determination of hydrogen peroxide and nitrite
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
الکتروشیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this work, a novel amperometric biosensor of hydrogen peroxide (H2O2) was developed based on the immobilization of myoglobin (Mb) on the surface of the multi-walled carbon nanotube (MWCNT) -Nafion-cysteamine (CA) modified gold electrode (Au) and its electrocatalytic activity was used for the determination of nitrite (NO2â). In the optimization studies, the best MWCNT and myoglobin amount were investigated. It was discovered at the experiments for the optimization of the working conditions that the buffer at this study as 50.0 mM, pH 7.0 phosphate buffer (PBS) and working temperature as 30 °C for the H2O2 biosensor. It was determined at the characterization studies on the biosensor that linear results are obtained between the ranges of 0.1 μM to 70.0 μM for H2O2 concentration and 1-250 μM for NO2â. The reproducibility of the biosensor was determined both H2O2 and nitrite. From the experiments, average value, standard deviation (SD) and coefficients of variation (CV%) were calculated to be 10.02 ± 0.43 μM, and 4.29% for 10.0 μM H2O2 (n = 6) and 52.0 ± 2.1 μM, and 3.89% for 50.0 μM nitrite (n = 8), respectively. At the same time the sample was analyzed for NO2â in drinking and mineral waters.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioelectrochemistry - Volume 101, February 2015, Pages 126-131
Journal: Bioelectrochemistry - Volume 101, February 2015, Pages 126-131
نویسندگان
Erhan Canbay, Berika Åahin, Müge Kıran, Erol Akyilmaz,