کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7830 566 2011 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
SPARC-derived protease substrates to enhance the plasmin sensitivity of molecularly engineered PEG hydrogels
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
SPARC-derived protease substrates to enhance the plasmin sensitivity of molecularly engineered PEG hydrogels
چکیده انگلیسی

Bioactive hydrogels formed from the Michael-type addition reactions of end-functionalized poly (ethylene glycol) macromers with thiol-containing protease-sensitive peptide crosslinkers have previously been described as matrices for cell-induced enzymatic remodeling. In this study, we sought to develop materials formulations with different degradation profiles by evaluating peptides derived from secreted protein acidic and rich in cysteine (SPARC) as potential substrates for plasmin, matrix metalloproteinase (MMP)-1, and MMP-2. Michaelis–Menten analysis showed that different peptides could provide a range of kcat values for each enzyme. In most cases, hydrogels formed with crosslinker peptides that had higher kcat values degraded faster when exposed to the appropriate enzyme(s), and fibroblasts showed increased cell proliferation and cell spreading when cultured in the faster degrading hydrogels. Further, greater cell invasion was observed from aortic ring segments embedded in the faster degrading hydrogels. The addition of the SPARC-derived peptides to the repertoire of protease-sensitive crosslinkers increases the potential application of these materials by providing enhanced susceptibility to plasmin. Further, the graded increases in kcat and the differential responses for plasmin, MMP-1, and MMP-2 can be used to engineer hydrogels with degradation properties tuned to the enzymes produced by particular cell types, allowing for broader in vivo application.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 32, Issue 5, February 2011, Pages 1301–1310
نویسندگان
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