کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7853 567 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The non-covalent decoration of self-assembling protein fibers
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
The non-covalent decoration of self-assembling protein fibers
چکیده انگلیسی

The design of self-assembling fibers presents challenges in basic science, and has potential for developing materials for applications in areas such as tissue engineering. A contemporary issue in the field is the construction of multi-component, functionalized systems. Previously, we have developed peptide-based fibers, the SAF system, that comprises two complementary peptides, which affords considerable control over assembly and morphology. Here we present a straightforward route to functionalizing the SAFs with small molecules and, subsequently, other moieties. This is achieved via non-covalent recruitment of charged peptide tags, which offers advantages such as further control, reversibility, and future prospects for developing recombinant tags. We demonstrate the concept by appending fluorescent labels and biotin (and thence gold nanoparticles) to the peptides, and visualising the resulting decorated SAFs by light and electron microscopy. The peptide tags bind in the nm–μm range, and show specificity compared with control peptides, and for the SAFs over similar α-helix-based peptide fibers.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 31, Issue 29, October 2010, Pages 7468–7474
نویسندگان
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