کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7869233 | 1509165 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Poly(glycidyl methacrylate-co-3-thienylmethylmethacrylate) as an immobilization matrix for microbial glycerol biosensing based on Gluconobacter oxydans
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
بیومتریال
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A commercial strain of Gluconobacter oxydans together with a new co-polymer Poly(glycidyl methacrylate-co-3-thienylmethylmethacrylate) (Poly(GMA-co-MTM)), which provides effective immobilization in the continuous flow system, was used in the sensor design. By taking the advantages of the nano-technology, carbon nanotubes (CNTs) were also added to the cell film and the sensitivity of the sensor was increased about 15 times. During the glycerol analysis in the continuous system, it was shown that composite film was not removed from the electrode surface and film elements were not washed out from the system. Glycerol analyses were performed by using batch loaded continuously flow cell at different flow rates of 1, 2, 4, and 6Â mL/min. The linear range was found as 2-100Â mM with the detection limit (LOD) of 0.057Â mM according to S/NÂ =Â 3. The calibration graphs were obtained for Poly(GMA-co-MTM)/G. oxydans and Poly(GMA-co-MTM)/CNT/G. oxydans biofilm electrodes in FIA mode, and sensitivities were found to be 1.50Â nA/mM and 19.13Â nA/mM, respectively. In this study, Poly(GMA-co-MTM) was used for the first time as a microbial matrix and was shown to be an effective immobilization agent.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 56, 1 November 2015, Pages 432-437
Journal: Materials Science and Engineering: C - Volume 56, 1 November 2015, Pages 432-437
نویسندگان
Tülay Ergön-Can, Elif Erhan, Ãmer Faruk Algur,