کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7869541 | 1509169 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Resorption of monetite calcium phosphate cement by mouse bone marrow derived osteoclasts
ترجمه فارسی عنوان
جذب سمان کلسیم فسفات مونیتیت توسط استئوکلاست های مشتق شده از مغز استخوان
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کلمات کلیدی
پرکننده استخوان، سیمان کلسیم فسفات اسید، ماکروفاژهای مغز استخوان، جذب استئوکلاستیک،
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
بیومتریال
چکیده انگلیسی
Recently the interest for monetite based biomaterials as bone grafts has increased; since in vivo studies have demonstrated that they are degradable, osteoconductive and improve bone healing. So far osteoclastic resorption of monetite has received little attention. The current study focuses on the osteoclastic resorption of monetite cement using primary mouse bone marrow macrophages, which have the potential to differentiate into resorbing osteoclasts when treated with receptor activator NF-κB ligand (RANKL). The osteoclast viability and differentiation were analysed on monetite cement and compared to cortical bovine bone discs. After seven days live/dead stain results showed no significant difference in viability between the two materials. However, the differentiation was significantly higher on the bone discs, as shown by tartrate resistant acid phosphatase (TRAP) activity and Cathepsin K gene expression. Moreover monetite samples with differentiated osteoclasts had a 1.4 fold elevated calcium ion concentration in their culture media compared to monetite samples with undifferentiated cells. This indicates active resorption of monetite in the presence of osteoclasts. In conclusion, this study suggests that osteoclasts have a crucial role in the resorption of monetite based biomaterials. It also provides a useful model for studying in vitro resorption of acidic calcium phosphate cements by primary murine cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 52, 1 July 2015, Pages 212-218
Journal: Materials Science and Engineering: C - Volume 52, 1 July 2015, Pages 212-218
نویسندگان
M. Montazerolghaem, M. Karlsson Ott, H. Engqvist, H. Melhus, A.J. Rasmusson,