Effect of calcination parameters on behavior of bone hydroxyapatite in artificial saliva and its biosafety

Being the main inorganic constitution of hard tissues (bone and teeth), calcium phosphates have been attractive in medical and dental applications in hard tissue repair. The evaluation of predicted osteointegration of implant material with bone most often begins with in vitro tests simulating living organism environment. The present study aims to present the behaviour in artificial saliva of hydroxyapatite (HAp) obtained from pork bone sludge using pioneer method and its features related to biosafety. Novel, waste-free HAp natural origin preparation method has been developed. Evaluation of diverse parameters, including physicochemical properties by various characterization techniques (eg. X-ray diffraction (XRD), FTIR, SEM and EDS methods) and biological behaviour (immersion in artificial saliva, NRU and MTT in vitro cytotoxicity) of natural origin bioceramic material intended to dentistry applications was the main goal of the research. On the basis of bioactivity tests in in vitro conditions, it was found that hydroxyapatite of natural origin exhibits the composition stability during incubation in artificial saliva. Performed cytotoxicity tests revealed no cytotoxic effects.