Quantitative retardance imaging by means of quadri-wave lateral shearing interferometry for label-free fiber imaging in tissues

We describe the use of quantitative retardance imaging, previously introduced in Aknoun et al. (2015), as a label-free method for in situ tissue imaging and extra-cellular matrix fiber organization study. A technical improvement of this quantitative retardance imaging technique, based on the use of a high-resolution quadri-wave lateral shearing interferometer, is implemented to perform fast quantitative linear retardance and retardance measurements on biological tissues. The technique combines a stack of quantitative phase images with varying polarization excitation to create retardance and orientation images at 1 Hz. This technique gives information about local retardance and structure of anisotropic components. In addition to bringing morphological information on unstained biopsies, it can reveal tumors development grades thanks to specific fiber organization imaging. In this present study, we demonstrate how this information can be used as tumor associated signature. We show the capability of our approach on mouse skin and human breast tissues.