کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7986479 | 1515136 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simultaneous two color image capture for sub-diffraction localization fluorescence microscopy
ترجمه فارسی عنوان
همزمان دو تصویر رنگی برای میکروسکوپ فلورسانس محلی سازی دیفرانسیل
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
دانش مواد (عمومی)
چکیده انگلیسی
A sub-diffraction limit fluorescence localization microscope was constructed using a standard cooled 1.4 mega-pixel fluorescence charge-coupled device (CCD) camera to simultaneously resolve closely adjacent paired quantum dots on a flat surface with emissions of 540 and 630Â nm. The images of the overlapping Airy discs were analyzed to determine the center of the point spread function after noise reduction using Fourier transformation analysis. The Cartesian coordinates of the centers of the point spread functions were compared in serial images. Histograms constructed from serial images fit well to Gaussian functions for resolving two quantum dots separated by as little as 10Â nm in the x-y coordinates. Statistical analysis of multiple pairs validated discrimination of inter-fluorophore distances that vary by 10Â nm. The method is simple and developed for x-y resolution of dilute fluorophores on a flat surface, not serial z sectioning.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Micron - Volume 80, January 2016, Pages 14-19
Journal: Micron - Volume 80, January 2016, Pages 14-19
نویسندگان
Ben J. Glasgow, Lie Ma,