کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8015112 1517174 2018 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels
ترجمه فارسی عنوان
کانی سازی آنزیمی هیدروژل غنی شده با کیتوزان با فیتاز
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد فناوری نانو (نانو تکنولوژی)
چکیده انگلیسی
Hydrogels mineralized with calcium phosphate (CaP) are increasingly popular bone regeneration biomaterials. Mineralization can be achieved by phosphatase enzyme incorporation and incubation in calcium glycerophosphate (CaGP). Gellan gum (GG) hydrogels containing the enzyme phytase and chitosan oligomer were mineralized in CaGP solution and characterized with human osteoblast-like MG63 cells and adipose tissue-derived stem cells (ADSC). Phytase induced CaP formation. Chitosan concentration determined mineralization extent and hydrogel mechanical reinforcement. Phytase-induced mineralization promoted MG63 adhesion and proliferation, especially in the presence of chitosan, and was non-toxic to MG63 cells (with and without chitosan). ADSC adhesion and proliferation were poor without mineralization. Chitosan did not affect ADSC osteogenic differentiation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Letters - Volume 214, 1 March 2018, Pages 186-189
نویسندگان
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