Facile synthesis of magnetic mesoporous titania and its application in selective and rapid enrichment of phosphopeptides

In this paper, a novel magnetic mesoporous titania composite (MNPs/mTiO2) was facilely prepared by anchoring magnetic nanoparticles (MNPs) onto the external surface of mesoporous titania (mTiO2) Ã¥sub-microparticles with the use of ethylenebis(nitrilodimethylene)tetraphosphonic acid (EDTMPA) as “bridge” molecule. The usefulness of MNPs/mTiO2 as adsorbent for the selective isolation and enrichment of phosphopeptides with subsequent analysis by matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF MS) was studied. The results showed that the MNPs/mTiO2 composite could be used in the highly efficient and rapid enrichment of phosphopeptides.